|Fluorescent 1 and 2 cell embryos|
|A single ES cell colony expressing Green Fluorescent Protein growing on a fibroblast feeder layer|
|Microinjection of embryonic stem cells into a blastocyst|
Dr, Transgenic Core Head
Genetically modified models represent one of the most powerful methods of functional gene analysis in vivo. Furthermore, the ability to introduce specific mutations into the genome enables models of human disease to be generated, facilitating insights into the pathophysiology of disease and providing a model with which therapeutic strategies and diagnostic tools can be optimized.
Our group provides groups within Oxford University access to transgenic technologies both on a fee-for-service type arrangement and on a collaborative basis. Technologies offered include embryo microinjection, embryonic stem cell transfection, Knock-out/-in construct design and in vivo shRNA mediated gene Knock-down. In addition, embryo rederivation and cryoconservation services are offered to facilitate the management, transfer and security of genetically modified strains.
The research activity of the group is focused on the development of novel methodologies for the generation of genetically modified models. The aims being to improve the reliability of the technology and to reduce the animal cost of research involving genetically modified models.
Phenotype of a transient neonatal diabetes point mutation (SUR1-R1183W) in mice
Sachse G. et al, (2020), Wellcome Open Research, 5, 15 - 15
A high-resolution map of non-crossover events reveals impacts of genetic diversity on mammalian meiotic recombination
Li R. et al, (2019), Nature Communications, 10
Loss of ZnT8 function protects against diabetes by enhanced insulin secretion.
Dwivedi OP. et al, (2019), Nat Genet
Genetic diversity and its unexpected impacts on recombination, genome evolution, speciation and sterility in mammals
Li R. et al, (2019), EUROPEAN JOURNAL OF HUMAN GENETICS, 27, 1073 - 1073
Gene editing - past, present and future
Davies B., (2019), HUMAN REPRODUCTION, 34, 1 - 1