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Bovine enterovirus (BEV) strain VG-5-27 derived from an infectious cDNA clone has been crystallized as extended hexagonal plates. Virus recovered from crystals produced cytopathic effect in BHK cells. These crystals diffract X-rays from high energy synchrotron sources to beyond 2.7 A. The crystal system is monoclinic, space group P2(1) with unit cell dimensions a = 388 A, b = 390 A, c = 360 A, beta = 113 degrees. The virion is 300 A in diameter and one whole particle constitutes the crystallographic asymmetric unit, giving rise to 60-fold non-crystallographic redundancy.

Original publication

DOI

10.1006/jmbi.1993.1339

Type

Journal article

Journal

J Mol Biol

Publication Date

05/06/1993

Volume

231

Pages

930 - 932

Keywords

Animals, Cattle, Cells, Cultured, Enterovirus, X-Ray Diffraction