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Two systems utilizing extracts derived from nuclei of adenovirus-infected cells which synthesize adenovirus DNA in vitro were analysed for indications of ADP-ribosylation of virus proteins. On incubation with [32P]NAD or [14C]NAD, modification of the adenovirus T antigen could be demonstrated in one of these systems. ADP-ribosylation of adenovirus core proteins V and VII could also be demonstrated with both nuclear extracts. However, using 3-aminobenzamide, a specific inhibitor of poly(ADP-ribose) polymerase, there was no evidence either in vivo or in vitro that ADP-ribosylation played a critical role in the replication process.

Original publication

DOI

10.1099/0022-1317-64-2-477

Type

Journal article

Journal

J Gen Virol

Publication Date

02/1983

Volume

64 (Pt 2)

Pages

477 - 483

Keywords

Adenosine Diphosphate Ribose, Adenoviridae, Antigens, Viral, Antigens, Viral, Tumor, Benzamides, DNA Replication, DNA, Viral, HeLa Cells, Humans, In Vitro Techniques, Nucleoside Diphosphate Sugars, Viral Core Proteins, Viral Proteins, Virus Replication