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The quest to develop an effective malaria vaccine remains a major priority in the fight against global infectious disease. An approach with great potential is a transmission-blocking vaccine which induces antibodies that prevent establishment of a productive infection in mosquitos that feed on infected humans, thereby stopping the transmission cycle. One of the most promising targets for such a vaccine is the gamete surface protein, Pfs48/45. Here we establish a system for production of full-length Pfs48/45 and use this to raise a panel of monoclonal antibodies. We map the binding regions of these antibodies on Pfs48/45 and correlate the location of their epitopes with their transmission-blocking activity. Finally, we present the structure of the C-terminal domain of Pfs48/45 bound to the most potent transmission-blocking antibody, and provide key molecular information for future structure-guided immunogen design.

Original publication

DOI

10.1038/s41467-018-06340-9

Type

Journal article

Journal

Nat Commun

Publication Date

20/09/2018

Volume

9

Keywords

Animals, Antibodies, Blocking, Antibodies, Monoclonal, Epitopes, Immunization, Malaria, Malaria Vaccines, Membrane Glycoproteins, Mice, Protein Domains, Protein Interaction Mapping, Protozoan Proteins