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We describe the characterization of sialic acid-binding Ig-like lectin-7 (siglec-7), a novel member of the siglec subgroup of the immunoglobulin superfamily. A full-length cDNA encoding siglec-7 was isolated from a human primary dendritic cell cDNA library. Siglec-7 is predicted to contain three extracellular immunoglobulin-like domains that comprise an N-terminal V-set domain and two C2-set domains, a transmembrane region and a cytoplasmic tail containing two tyrosine residues embodied in immunoreceptor tyrosine-based inhibition motif-like motifs. Overall, siglec-7 exhibited a high degree of sequence similarity to genes encoding CD33 (siglec-3), siglec-5, OBBP1/siglec-6, and OBBP-like protein and mapped to the same region on chromosome 19q13.3. When siglec-7 was expressed on COS or Chinese hamster ovary cells, it was able to mediate high levels of sialic acid-dependent binding to human erythrocytes and soluble sialoglycoconjugates, suggesting that it may be involved in cell-cell interactions. Among human peripheral blood leukocytes, siglec-7 was found to be present at low levels on granulocytes, intermediate levels on monocytes, and relatively high levels on a major subset of natural killer cells and a minor subset of CD8(+) T cells. Immunoprecipitation experiments indicated that siglec-7 is expressed as a monomer of approximately 65 kDa.

Type

Journal article

Journal

J Biol Chem

Publication Date

26/11/1999

Volume

274

Pages

34089 - 34095

Keywords

3T3 Cells, Amino Acid Sequence, Animals, Antigens, CD, Antigens, Differentiation, Myelomonocytic, Blotting, Northern, CHO Cells, COS Cells, Cell Line, Chromosome Banding, Chromosome Mapping, Chromosomes, Human, Pair 9, Cricetinae, DNA, Complementary, Female, Gene Expression, HL-60 Cells, Humans, Immunophenotyping, In Situ Hybridization, Fluorescence, Killer Cells, Natural, Lectins, Leukocytes, Mononuclear, Male, Mice, Molecular Sequence Data, Molecular Weight, Monocytes, RNA, Messenger, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Tissue Distribution, Tumor Cells, Cultured