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The Fanconi Anemia (FA) pathway is important for repairing interstrand crosslinks (ICLs) between the Watson-Crick strands of the DNA double helix. An initial and essential stage in the repair process is the detection of the ICL. Here, we report the identification of UHRF2, a paralogue of UHRF1, as an ICL sensor protein. UHRF2 is recruited to ICLs in the genome within seconds of their appearance. We show that UHRF2 cooperates with UHRF1, to ensure recruitment of FANCD2 to ICLs. A direct protein-protein interaction is formed between UHRF1 and UHRF2, and between either UHRF1 and UHRF2, and FANCD2. Importantly, we demonstrate that the essential monoubiquitination of FANCD2 is stimulated by UHRF1/UHRF2. The stimulation is mediating by a retention of FANCD2 on chromatin, allowing for its monoubiquitination by the FA core complex. Taken together, we uncover a mechanism of ICL sensing by UHRF2, leading to FANCD2 recruitment and retention at ICLs, in turn facilitating activation of FANCD2 by monoubiquitination.

Original publication

DOI

10.1371/journal.pgen.1007643

Type

Journal article

Journal

PLoS Genet

Publication Date

10/2018

Volume

14

Keywords

Amino Acid Sequence, CCAAT-Enhancer-Binding Proteins, Cell Line, Cell Nucleus, Chromatin, DNA, DNA Damage, DNA Repair, Fanconi Anemia, Fanconi Anemia Complementation Group D2 Protein, Fanconi Anemia Complementation Group Proteins, HEK293 Cells, HeLa Cells, Humans, Protein Interaction Domains and Motifs, Ubiquitin-Protein Ligases, Ubiquitination