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<jats:p>Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses.</jats:p>

Original publication

DOI

10.1128/jvi.02261-13

Type

Journal article

Journal

Journal of Virology

Publisher

American Society for Microbiology

Publication Date

01/12/2013

Volume

87

Pages

12667 - 12674