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We have examined the genetic aberrations in two near-diploid glioblastoma multiforme cell lines that appear to have arisen from different glial lineages. One cell line, Hu-O-2A/Gb1, expresses antigens and metabolic profiles characteristic of the oligodendrocyte-type-2 astrocyte (0-2A) lineage of the rat central nervous system. This line generates, in vitro, cells with characteristics of 0-2A progenitor cells, oligodendrocytes and astrocytes. The second cell line, IN1434, is derived from an astrocyte or a precursor cell restricted to astrocytic differentiation. In Hu-O-2A/Gb1 the sole homologue of chromosome 10 is disrupted at band 10p11-12.1 by translocation with chromosomes X and 15. The translocation breakpoint is localized between genetic markers D10S2103 and [D10S637, D10S1962, D10S355]. Other aberrations include a 5;14 translocation, deletion of the long and short arms of chromosome 16 and loss of one copy of the CDKN2 gene. IN1434 cells share some cytogenetic abnormalities with Hu-O-2A/Gb1 cells, despite their apparent derivation from a different biological origin, but also have translocations involving the long and short arms of chromosome 1 and the long arm of chromosome 7, and deletion of chromosome 13 at bands 13q12-21.

Original publication

DOI

10.1038/sj.bjc.6690116

Type

Journal article

Journal

Br J Cancer

Publication Date

02/1999

Volume

79

Pages

724 - 731

Keywords

Animals, Astrocytes, Brain Neoplasms, Cell Line, Chromosome Aberrations, Chromosome Mapping, Chromosomes, Human, Pair 10, Glioblastoma, Humans, Karyotyping, Male, Microsatellite Repeats, Middle Aged, Oligodendroglia, Rats, Translocation, Genetic, Tumor Cells, Cultured