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This introductory chapter provides a brief historical survey of the key elements incorporated into commonly used E. coli-based expression systems. The highest impact in expression technology is associated with innovations that were based on extensively studied biological systems, and where the tools were widely distributed in the academic community.

Original publication

DOI

10.1007/978-1-4939-6887-9_1

Type

Chapter

Publication Date

2017

Volume

1586

Pages

3 - 10

Keywords

E. coli, Expression vectors, Promoter, Protein engineering, Recombinant protein, Cloning, Molecular, Escherichia coli, Gene Expression, Genetic Vectors, Promoter Regions, Genetic, Protein Engineering, Recombinant Proteins