Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Mutations in the human MMAA gene cause the metabolic disorder cblA-type methylmalonic aciduria (MMA), although knowledge of the mechanism of dysfunction remains lacking. MMAA regulates the incorporation of the cofactor adenosylcobalamin (AdoCbl), generated from the MMAB adenosyltransferase, into the destination enzyme methylmalonyl-CoA mutase (MUT). This function of MMAA depends on its GTPase activity, which is stimulated by an interaction with MUT. Here, we present 67 new patients with cblA-type MMA, identifying 19 novel mutations. We biochemically investigated how missense mutations in MMAA in 22 patients lead to disease. About a third confer instability to the recombinant protein in bacterial and human expression systems. All 15 purified mutant proteins demonstrated wild-type like intrinsic GTPase activity and only one (p.Asp292Val), where the mutation is in the GTP binding domain, revealed decreased GTP binding. However, all mutations strongly decreased functional association with MUT by reducing GTPase activity stimulation upon incubation with MUT, while nine mutant proteins additionally lost the ability to physically bind MUT. Finally, all mutations interfered with gating the transfer of AdoCbl from MMAB to MUT. This work suggests loss of functional interaction between MMAA and MUT as a disease-causing mechanism that impacts processing and assembly of a cofactor to its destination enzyme.

Original publication

DOI

10.1002/humu.23251

Type

Journal article

Journal

Hum Mutat

Publication Date

08/2017

Volume

38

Pages

988 - 1001

Keywords

MMAA, cblA-type methylmalonic aciduria, methylmalonyl-CoA mutase, missense variants, patient cohort, protein-protein interaction, vitamin B12, Amino Acid Metabolism, Inborn Errors, Child, Child, Preschool, Cobamides, Female, Genotype, Humans, Infant, Infant, Newborn, Male, Membrane Transport Proteins, Methylmalonyl-CoA Mutase, Mitochondrial Proteins, Mutation, Mutation, Missense, Protein Binding