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Plasmodium vivax is the most widely distributed malaria species and the most prevalent species of malaria in America and Asia. Vaccine development against P. vivax is considered a priority in the global program for the eradication of malaria. Earlier studies have characterized the Apical Membrane Antigen 1 (AMA-1) ectodomain and the C-terminal region (19kDa) of the Merozoite Surface Protein 1 (MSP-1) of P. vivax as immunodominant antigens. Based on this characterization, we designed a chimeric recombinant protein containing both merozoite immunodominant domains (PvAMA166-MSP119). The recombinant PvAMA166-MSP119 was successfully expressed in Pichia pastoris and used to immunize two different mouse strains (BALB/c and C57BL/6) in the presence of the Poly (I:C) as an adjuvant. Immunization with the chimeric protein induced high antibody titers against both proteins in both strains of mice as detected by ELISA. Antisera also recognized the native proteins expressed on the merozoites of mature P. vivax schizonts. Moreover, this antigen was able to induce IFN-gamma-secreting cells in C57BL/6 mice. These findings indicate that this novel yeast recombinant protein containing PvAMA166 and PvMSP119 is advantageous, because of improved antibody titers and cellular immune response. Therefore, this formulation should be further developed for pre-clinical trials in non-human primates as a potential candidate for a P. vivax vaccine.

Original publication

DOI

10.1016/j.vaccine.2017.03.023

Type

Journal article

Journal

Vaccine

Publication Date

25/04/2017

Volume

35

Pages

2463 - 2472

Keywords

Malaria, Plasmodium vivax, Recombinant vaccine, Adjuvants, Immunologic, Animals, Antibodies, Protozoan, Antigens, Protozoan, Enzyme-Linked Immunosorbent Assay, Female, Gene Expression, Interferon-gamma, Leukocytes, Mononuclear, Malaria Vaccines, Membrane Proteins, Merozoite Surface Protein 1, Mice, Inbred BALB C, Mice, Inbred C57BL, Pichia, Plasmodium vivax, Poly I-C, Protozoan Proteins, Recombinant Fusion Proteins, Vaccines, Synthetic