Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Interferon-gamma (IFN-gamma) is a pleiotrophic cytokine with immunomodulatory effects on a variety of immune cells. Mice with a targeted disruption of the IFN-gamma gene were generated. These mice developed normally and were healthy in the absence of pathogens. However, mice deficient in IFN-gamma had impaired production of macrophage antimicrobial products and reduced expression of macrophage major histocompatibility complex class II antigens. IFN-gamma-deficient mice were killed by a sublethal dose of the intracellular pathogen Mycobacterium bovis. Splenocytes exhibited uncontrolled proliferation in response to mitogen and alloantigen. After a mixed lymphocyte reaction, T cell cytolytic activity was enhanced against allogeneic target cells. Resting splenic natural killer cell activity was reduced in IFN-gamma-deficient mice. Thus, IFN-gamma is essential for the function of several cell types of the murine immune system.

Type

Journal article

Journal

Science

Publication Date

19/03/1993

Volume

259

Pages

1739 - 1742

Keywords

Animals, Cell Division, Cytotoxicity, Immunologic, Histocompatibility Antigens Class II, Immunity, Interferon-gamma, Isoantigens, Killer Cells, Natural, Lymphocyte Culture Test, Mixed, Macrophages, Mice, Mice, Inbred C57BL, Mice, Transgenic, Mutation, Mycobacterium bovis, Nitric Oxide, Spleen, T-Lymphocytes, Transfection, Tuberculosis