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Rapid-sedimenting DNA-membrane complexes were obtained from both Bacillus subtilis and Escherichia coli by a method involving gentle lysis followed by restriction enzyme digestion and sucrose gradient fractionation. These complexes were substantially enriched in chromosomal origin DNA, and in B. subtilis, the complexes were enriched in penicillin-binding proteins relative to that of the total membrane. Such complexes may represent procaryotic membrane domains which are topographically and functionally distinct.

Type

Journal article

Journal

J Bacteriol

Publication Date

10/1985

Volume

164

Pages

192 - 200

Keywords

Bacillus subtilis, Bacterial Proteins, Carboxypeptidases, Carrier Proteins, Cell Membrane, Chromosomes, Bacterial, DNA, Bacterial, Escherichia coli, Hexosyltransferases, Molecular Weight, Muramoylpentapeptide Carboxypeptidase, Penicillin-Binding Proteins, Peptidyl Transferases