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We previously described different cytotoxic T lymphocyte (CTL) clones isolated from the blood lymphocytes of a melanoma patient after in vitro stimulation with autologous tumor cells. These CTL clones recognized at least 2 distinct antigens on the melanoma cells. Here, we show that one of them consists of a peptide derived from tyrosinase and presented by HLA-B35. The peptide is 9 amino acids long and has the sequence LPSSADVEF. It can be presented by the 2 major B35 allelic subtypes, B*3501 and B*3503. As HLA-B35 is one of the most frequent HLA-B specificities, being present in about 20% of Caucasian individuals, it may be a useful target for peptide-based immunotherapy of melanoma.

Type

Journal article

Journal

Int J Cancer

Publication Date

10/12/1999

Volume

83

Pages

755 - 759

Keywords

Alleles, Amino Acid Sequence, Animals, B-Lymphocytes, COS Cells, Cell Line, Transformed, Cytotoxicity, Immunologic, HLA-B35 Antigen, Herpesvirus 4, Human, Humans, Melanoma, Monophenol Monooxygenase, Peptide Fragments, Recombinant Proteins, T-Lymphocytes, Cytotoxic, Transfection, Tumor Cells, Cultured