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The use of methylmalonyl-CoA epimerase (MCEE) to improve stereoselectivity in crotonase-mediated biocatalysis is exemplified by the coupling of MCEE, crotonyl-CoA carboxylase reductase and carboxymethylproline synthase in a three-enzyme one-pot sequential synthesis of functionalised C-5 carboxyalkylprolines starting from crotonyl-CoA and carbon dioxide.

Original publication

DOI

10.1002/cbic.201500644

Type

Journal article

Journal

Chembiochem

Publication Date

15/03/2016

Volume

17

Pages

471 - 473

Keywords

antibiotics, biosynthesis, carbapenem, crotonases, dynamic kinetic discrimination, enolate reactivity, stereoselectivity, Enoyl-CoA Hydratase, Racemases and Epimerases, Stereoisomerism