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A method for meningococcal X (MenX) polysaccharide quantification by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) is described. The polysaccharide is hydrolyzed by strong acidic treatment, and the peak of glucosamine-4-phosphate (4P-GlcN) is detected and measured after chromatography. In the selected conditions of hydrolysis, 4P-GlcN is the prevalent species formed, with GlcN detected for less than 5% in moles. As standard for the analysis, the monomeric unit of MenX polysaccharide, N-acetylglucosamine-4-phosphate (4P-GlcNAc), was used. This method for MenX quantification is highly selective and sensitive, and it constitutes an important analytical tool for the development of a conjugate vaccine against MenX.

Original publication

DOI

10.1016/j.ab.2013.08.001

Type

Journal article

Journal

Anal Biochem

Publication Date

15/11/2013

Volume

442

Pages

259 - 261

Keywords

Carbohydrate-based vaccine, Meningococcal serogroup X polysaccharide, Quantitative estimation, Acetylglucosamine, Chromatography, High Pressure Liquid, Chromatography, Ion Exchange, Electrochemistry, Glucosephosphates, Polysaccharides, Bacterial, Reference Standards