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We describe a "gel-assisted" proteomic sample preparation method for MS analysis. Solubilized protein extracts or intact cells are copolymerized with acrylamide, facilitating denaturation, reduction, quantitative cysteine alkylation, and matrix formation. Gel-aided sample preparation has been optimized to be highly flexible, scalable, and to allow reproducible sample generation from 50 cells to milligrams of protein extracts. This methodology is fast, sensitive, easy-to-use on a wide range of sample types, and accessible to nonspecialists.

Original publication

DOI

10.1002/pmic.201400436

Type

Journal article

Journal

Proteomics

Publication Date

04/2015

Volume

15

Pages

1224 - 1229

Keywords

Bottom-up proteomics, Comparative proteomics, Gel-assisted sample preparation, Sample preparation, Technology, Acrylic Resins, Cell Extracts, Cells, Cultured, Humans, Proteome, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization