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BACKGROUND: Phage-encoded serine integrases, such as φC31 integrase, are widely used for genome engineering. Fifteen such integrases have been described but their utility for genome engineering has not been compared in uniform assays. RESULTS: We have compared fifteen serine integrases for their utility for DNA manipulations in mammalian cells after first demonstrating that all were functional in E. coli. Chromosomal recombination reporters were used to show that seven integrases were active on chromosomally integrated DNA in human fibroblasts and mouse embryonic stem cells. Five of the remaining eight enzymes were active on extra-chromosomal substrates thereby demonstrating that the ability to mediate extra-chromosomal recombination is no guide to ability to mediate site-specific recombination on integrated DNA. All the integrases that were active on integrated DNA also promoted DNA integration reactions that were not mediated through conservative site-specific recombination or damaged the recombination sites but the extent of these aberrant reactions varied over at least an order of magnitude. Bxb1 integrase yielded approximately two-fold more recombinants and displayed about two fold less damage to the recombination sites than the next best recombinase; φC31 integrase. CONCLUSIONS: We conclude that the Bxb1 and φC31 integrases are the reagents of choice for genome engineering in vertebrate cells and that DNA damage repair is a major limitation upon the utility of this class of site-specific recombinase.

Original publication

DOI

10.1186/1472-6750-13-87

Type

Journal article

Journal

BMC Biotechnol

Publication Date

20/10/2013

Volume

13

Keywords

Amino Acid Sequence, Animals, Attachment Sites, Microbiological, Bacteriophages, Cell Line, Tumor, Cloning, Molecular, DNA, DNA Nucleotidyltransferases, Embryonic Stem Cells, Escherichia coli, Fibrosarcoma, Gene Deletion, Genome, Human, Humans, Integrases, Mice, Molecular Sequence Data, Plasmids, Recombinases, Recombination, Genetic, Serine