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Real-time PCR is widely employed to quantify PCR products across a range of applications. However, accurate real-time PCR is not always technically feasible, and alternative methods for PCR product quantification can be expensive and time consuming to validate. We have developed an inexpensive, rapid, and immediately accessible protocol to quantify PCR products, by measuring ethidium bromide fluorescence of PCR products excised from agarose gels. This protocol has relevance to a broad range of methods in molecular biology where quantification of PCR products is necessary.

Original publication

DOI

10.1016/j.ab.2013.11.003

Type

Journal article

Journal

Anal Biochem

Publication Date

15/02/2014

Volume

447

Pages

30 - 32

Keywords

Ethidium bromide, Fluorescence, PCR, Quantification, Base Sequence, DNA, Ethidium, HEK293 Cells, Humans, Polymerase Chain Reaction, Spectrometry, Fluorescence