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A reversed-phase liquid chromatography-linear ion trap-Fourier transform ion cyclotron resonance-mass spectrometric method was developed for the profiling of lipids in human and mouse plasma. With the use of a fused-core C 8 column and a binary gradient, more than 160 lipids belonging to eight different classes were detected in a single LC-MS run. The method was fully validated and the analytical characteristics such as linearity ( R (2), 0.994-1.000), limit of detection (0.08-1.28 microg/mL plasma), repeatability (RSD, 2.7-7.9%) and intermediate precision (RSD, 2.7-15.6%) were satisfactory. The method was successfully applied to p53 mutant mice plasma for studying some phenotypic effects of p53 expression.

Original publication

DOI

10.1021/pr800373m

Type

Journal article

Journal

J Proteome Res

Publication Date

11/2008

Volume

7

Pages

4982 - 4991

Keywords

Animals, Chromatography, Liquid, Cyclotrons, Lipids, Mice, Mice, Mutant Strains, Reproducibility of Results, Spectroscopy, Fourier Transform Infrared, Tandem Mass Spectrometry, Tumor Suppressor Protein p53