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Successful cryogenic X-ray structure determination from a single high-pressure-frozen bovine enterovirus 2 crystal is reported. The presented high-pressure-freezing procedure is based on a commercially available device and allows the cryocooling of macromolecular crystals directly in their mother liquor without the time- and crystal-consuming search for optimal cryoconditions. The method is generally applicable and will allow cryogenic data collection from all types of macromolecular crystals.

Original publication

DOI

10.1107/S090744491204543X

Type

Journal article

Journal

Acta Crystallogr D Biol Crystallogr

Publication Date

02/2013

Volume

69

Pages

308 - 312

Keywords

cryocooling, high-pressure-freezing, virology, Animals, Cattle, Cryoelectron Microscopy, Cryoprotective Agents, Crystallization, Crystallography, X-Ray, Diffusion, Enterovirus, Bovine, Freezing, Pressure