Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

A new structural gene, carG, involved in the biosynthesis of carotenoids in the fungus Mucor circinelloides was isolated by heterologous hybridisation, using a probe derived from the Gibberella fujikuroi ggs1 gene. Functional analyses in Escherichia coli showed that the encoded protein has geranylgeranyl pyrophosphate (GGPP) synthase activity. A comparison of the deduced protein with other GGPP synthases suggested that the carG gene might have evolved from other larger genes present in some fungi. The analysis of carG mRNA accumulation after blue light irradiation showed that the expression of this gene is up-regulated by blue light, as happens with the other structural genes involved in carotenogenesis in M. circinelloides. Analysis of the promoter region revealed the presence of several APE-like sequences, which participate in the blue-light regulation of the expression of different fungal genes. These sequences are also present in the above-mentioned Mucor genes and strongly support the idea that this gene plays an important role in the regulation of carotenoid synthesis, despite belonging to a more general metabolic pathway.

Original publication

DOI

10.1007/s00294-003-0376-5

Type

Journal article

Journal

Curr Genet

Publication Date

05/2003

Volume

43

Pages

112 - 120

Keywords

Alkyl and Aryl Transferases, Base Sequence, Blotting, Northern, Blotting, Southern, Carotenoids, DNA Primers, Dimethylallyltranstransferase, Farnesyltranstransferase, Gene Expression Regulation, Fungal, Genetic Complementation Test, Light, Molecular Sequence Data, Mucor, Nucleic Acid Hybridization, Plasmids, Sequence Alignment, Sequence Analysis, DNA, Up-Regulation