Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Kindlin-3, a 75-kDa protein, has been shown to be critical for hemostasis, immunity, and bone metabolism via its role in integrin activation. The Kindlin family is hallmarked by a FERM domain comprised of F1, F2, and F3 subdomains together with an N-terminal F0 domain and a pleckstrin homology domain inserted in the F2 domain. Recombinant Kindlin-3 was cloned, expressed, and purified, and its domain organization was studied by x-ray scattering and other techniques to reveal an extended conformation. This unusual elongated structure is similar to that found in the paralogue Talin head domain. Analytical ultracentrifugation experiments indicated that Kindlin-3 forms a ternary complex with the Talin and β-integrin cytoplasmic tails. NMR showed that Kindlin-3 specifically recognizes the membrane-distal tail NPXY motif in both the β(1A) and β(1D) isoforms, although the interaction is stronger with β(1A). An upstream Ser/Thr cluster in the tails also plays a critical role. Overall these data support current biological, clinical, and mutational data on Kindlin-3/β-tail binding and provide novel insights into the overall conformation and interactions of Kindlin-3.

Original publication

DOI

10.1074/jbc.M112.415208

Type

Journal article

Journal

J Biol Chem

Publication Date

02/11/2012

Volume

287

Pages

37715 - 37731

Keywords

Amino Acid Motifs, Amino Acid Sequence, Animals, Biophysical Phenomena, Cytoskeletal Proteins, Integrin beta1, Magnetic Resonance Spectroscopy, Membrane Proteins, Mice, Mutation, Protein Binding, Protein Conformation, Protein Isoforms, Protein Structure, Tertiary, Protein Subunits, Recombinant Proteins, Scattering, Small Angle, Sf9 Cells, Talin, X-Ray Diffraction