Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

For many years the IFN-gamma ex vivo ELISPOT has been a major assay for assessing human T-cell responses generated by malaria vaccines. The ELISPOT assay is a sensitive assay, but an imperfect correlate of protection against malaria. Monokine induced by gamma (MIG), or CXCL9, is a chemokine induced by IFN-gamma and has the potential to provide amplification of the IFN-gamma signal. MIG secretion could provide a measure of bio-active IFN-gamma and a functional IFN-gamma signalling pathway. We report that detecting MIG by flow cytometry and by RT-PCR can be more sensitive than the detection of IFN-gamma using these methods. We also find that there is little inter-individual variability in MIG secretion when detected by flow cytometry and that the MIG assay may be used to estimate the amount of bio-active IFN-gamma present. Measurement of MIG alongside IFN-gamma may provide a fuller picture of Th1 type responses post-vaccination.

Original publication

DOI

10.1016/j.jim.2008.09.021

Type

Journal article

Journal

J Immunol Methods

Publication Date

01/01/2009

Volume

340

Pages

33 - 41

Keywords

Adolescent, Adult, Aged, Animals, Brefeldin A, Chemokine CXCL9, Female, Flow Cytometry, Humans, Interferon-gamma, Malaria Vaccines, Malaria, Falciparum, Male, Middle Aged, Plasmodium falciparum, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocytes, Vaccines, Synthetic, Young Adult