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The ability of the dendritic cell (DC) subsets, CD8alpha+ and CD8alpha- DCs, to initiate a CD8 T cell response or to activate memory CD8 T cells and generate effector CD8 T cells has been controversial. In this study, we analyse the capacity of splenic DC subsets to induce CD8 T cell responses to a CD8 T cell epitope (pb9) of a malaria antigen. The administration of peptide-pulsed CD8alpha- or CD8alpha+ DCs primes and boosts a primed CD8 T cell response against the malaria epitope. In vitro, depletion of CD11c(+) DCs from mouse splenocytes, immunised with recombinant vaccinia virus Ankara (MVA) expressing pb9 epitope, significantly reduced the generation of pb9-specific IFNgamma producing effector CD8 T cells, indicating that splenic DCs are involved in the development of pb9-specific IFNgamma producing effector cells. Taken together, this result shows that both DC subsets have the ability to prime and boost CD8 T cell responses and are involved in the activation of memory CD8 T cells.

Original publication

DOI

10.1016/j.cellimm.2004.03.010

Type

Journal article

Journal

Cell Immunol

Publication Date

03/2004

Volume

228

Pages

15 - 19

Keywords

Animals, CD8 Antigens, CD8-Positive T-Lymphocytes, Dendritic Cells, Female, Immunologic Memory, Interferon-gamma, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Spleen