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Currently, there are no reliable RBC invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin-treated P vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P vivax isolates freshly collected from patients in Thailand.

Original publication

DOI

10.1182/blood-2011-04-348748

Type

Journal article

Journal

Blood

Publication Date

29/09/2011

Volume

118

Pages

e74 - e81

Keywords

Cells, Cultured, Hematologic Tests, High-Throughput Screening Assays, Host-Pathogen Interactions, Humans, Malaria, Vivax, Plasmodium vivax, Reproducibility of Results, Reticulocytes