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Platelet-endothelial cell adhesion molecule-1 or CD31 (PECAM-1/CD31) is a receptor recognized by Plasmodium falciparum-parasitized erythrocytes (pRBCs). Fluorescence-labeled soluble recombinant PECAM-1/CD31 (sPECAM-1/CD31) is shown to bind to the surface of P. falciparum-infected erythrocytes on up to 70% of the cells. Binding is blocked by the addition of the unlabeled receptor in a dose-dependent fashion, but not by unrelated receptor-proteins. A significant correlation was found between the binding of sPECAM-1/CD31 to pRBCs and the binding to transfected L cells expressing the receptor as seen with six different P. falciparum lines or clones. Panning of cultures on PECAM-1/CD31 transfected L cells was paralleled by an increase in the binding of sPECAM-1/CD31. The pRBCs of 54% of fresh patient-isolates bound sPECAM-1/CD31 with a mean rate of 12.9% (range = 1.1-44%). The data suggest that PECAM-1/CD31 is a common receptor recognized by wild isolates and that the soluble PECAM-1/CD31 suspension assay is a sensitive and reliable way to study PECAM-1/CD31 binding.

Type

Journal article

Journal

Am J Trop Med Hyg

Publication Date

07/2001

Volume

65

Pages

47 - 51

Keywords

Animals, CHO Cells, COS Cells, Cell Adhesion, Child, Preschool, Cricetinae, Erythrocytes, Fluorescent Dyes, Humans, Hydrazines, Kenya, L Cells (Cell Line), Malaria, Falciparum, Mice, Microscopy, Fluorescence, Plasmodium falciparum, Platelet Endothelial Cell Adhesion Molecule-1, Recombinant Proteins, Solubility, Transfection