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CMV-specific memory CD8(+) T cells accumulate over time to reach high frequencies amongst peripheral blood lymphocytes - a phenomenon termed 'memory inflation'. Using tetramer staining on samples from a large number of subjects and multivariate regression analysis, we were able to relate this to the phenotype of CD8(+) T cells. We made the following observations: (i) CD85j (ILT-2/LIR-1) was highly expressed alongside CD57 - an established effector memory marker - on CMV-specific CD8(+) T cells; (ii) on CD8(+) T cells as a whole, with increasing age, CD57 and CD85j (ILT-2/LIR-1) expression increased whereas CCR7 expression decreased, indicating increasing maturation of the total CD8(+) T-cell compartment with age; (iii) unit increases in the percentage of CMV-specific CD8(+) T cells expressing CD57 and CD85j (ILT-2/LIR-1) were associated with incremental expansion of these T-cell populations; (iv) CMV seropositivity is associated with a marked effect on the overall phenotype of CD8(+) T cells (at any given age, CMV seropositivity is associated with an 18.7% increase in CD85j (ILT-2/LIR-1) expression); and (v) from our observations we estimated from this an apparent 'ageing effect' of CMV on CD8(+) T cells of 35.4 years. The data presented are consistent with a predictable, unidirectional and linear model of virus specific T-cell differentiation and maturation.

Original publication

DOI

10.1111/j.1440-1711.2005.01321.x

Type

Journal article

Journal

Immunol Cell Biol

Publication Date

04/2005

Volume

83

Pages

182 - 188

Keywords

Adolescent, Adult, Age Factors, Aged, Aged, 80 and over, Aging, Antigens, CD, Biomarkers, CD57 Antigens, CD8-Positive T-Lymphocytes, Cell Proliferation, Cytomegalovirus, Cytomegalovirus Infections, Humans, Immunologic Memory, Leukocyte Immunoglobulin-like Receptor B1, Middle Aged, Receptors, Immunologic