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Dialysate effluent from 41 patients presenting with 54 episodes of CAPD peritonitis was examined by four culture techniques, three employing methods for the lysis of peritoneal leucocytes. The most efficient method employed a distilled water lysis-centrifugation technique (81% of episodes culture-positive). Filtration (without leucocyte lysis) was less effective (74% culture-positive). The results of a mechanical leucocyte lysis technique were less satisfactory still, the culture positive rate of 74% being compromised by the recovery of the infecting organism in low numbers, and by an association with a high incidence of plate contamination. The results of a bile-salt lysis technique were the least satisfactory (67% culture-positive).

Original publication

DOI

10.1016/0195-6701(90)90115-5

Type

Journal article

Journal

The Journal of hospital infection

Publication Date

10/1990

Volume

16

Pages

263 - 269

Addresses

Department of Medical Microbiology, St Bartholemew's Hospital, London.

Keywords

Leukocytes, Humans, Bacteria, Peritonitis, Water, Bile Acids and Salts, Peritoneal Dialysis, Continuous Ambulatory, Centrifugation, Bacteriological Techniques