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We have examined the requirement for major histocompatibility complex (MHC)-restricted T-cell help in the secondary in vivo antibody response to phosphocholine (PC). The memory response to PC has been demonstrated previously to be comprised of T15-dominant IgM and IgG3 plaque-forming cells (PFC) derived primarily from the Lyb-5+ B-cell subset, and IgG1 and IgG2 PFC, few of which bear the T15 idiotype and are predominantly derived from the Lyb-5- B-cell subset. Using carrier-primed (A X B)F1 T cells which have matured in a parentA chimeric environment so that "self" recognition is of the MHC determinants of parentA but not parentB, we have found that parentA PC-primed B cells, but not parentB PC-primed B cells, are activated. Even in the presence of an ongoing parentA anti-PC response, parentB PC-primed B cells were not activated, indicating that the restriction was between the helper T cell and the B cell, not between T-helper and accessory cells. MHC-restricted T-cell help was required by B cells producing T15+ and T15- IgM, IgG3, IgG1, and IgG2 responses.

Original publication

DOI

10.1016/0008-8749(86)90055-9

Type

Journal article

Journal

Cellular immunology

Publication Date

07/1986

Volume

100

Pages

570 - 576

Keywords

B-Lymphocytes, T-Lymphocytes, Helper-Inducer, Cell Line, Animals, Mice, Inbred Strains, Mice, Inbred C57BL, Mice, Choline, Phosphorylcholine, Antigens, Differentiation, B-Lymphocyte, Immunoglobulin Idiotypes, Antigens, Surface, Lymphocyte Activation, Major Histocompatibility Complex, Immunologic Memory