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Photoactivated cross-linking of peptides to proteins is a useful strategy for identifying enzyme-substrate and protein-protein interactions in cell lysates as demonstrated by studies on the human hypoxia inducible factor system.

Original publication

DOI

10.1039/c0cc04457a

Type

Journal article

Journal

Chem Commun (Camb)

Publication Date

07/02/2011

Volume

47

Pages

1488 - 1490

Keywords

Cells, Cultured, Cross-Linking Reagents, Humans, Hypoxia-Inducible Factor 1, alpha Subunit, Kidney, Molecular Probes, Peptide Fragments, Photochemistry, Protein Interaction Mapping, Spectrometry, Mass, Electrospray Ionization, Von Hippel-Lindau Tumor Suppressor Protein