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A liquid chromatographic tandem mass spectroscopy method for the quantification of artemisinin in human heparinised plasma has been developed and validated. The method uses Oasis HLB mu-elution solid phase extraction 96-well plates to facilitate a high throughput of 192 samples a day. Artesunate (internal standard) in a plasma-water solution was added to plasma (50 microL) before solid phase extraction. Artemisinin and its internal standard artesunate were analysed by liquid chromatography and MS/MS detection on a Hypersil Gold C18 (100 mm x 2.1 mm, 5 microm) column using a mobile phase containing acetonitrile-ammonium acetate 10mM pH 3.5 (50:50, v/v) at a flow rate of 0.5 mL/min. The method has been validated according to published FDA guidelines and showed excellent performance. The within-day, between-day and total precisions expressed as R.S.D., were lower than 8% at all tested quality control levels including the upper and lower limit of quantification. The limit of detection was 0.257 ng/mL for artemisinin and the calibration range was 1.03-762 ng/mL using 50 microL plasma. The method was free from matrix effects as demonstrated both graphically and quantitatively.

Original publication

DOI

10.1016/j.jpba.2008.12.014

Type

Journal article

Journal

J Pharm Biomed Anal

Publication Date

05/04/2009

Volume

49

Pages

768 - 773

Keywords

Analysis of Variance, Antimalarials, Artemisinins, Artesunate, Biological Availability, Chemistry, Pharmaceutical, Chromatography, High Pressure Liquid, Humans, Indicators and Reagents, Reference Standards, Reproducibility of Results, Spectrometry, Mass, Electrospray Ionization, Tandem Mass Spectrometry